恒遠推薦：游離脂肪酸定量檢測試劑盒

恒遠推薦：游離脂肪酸定量檢測試劑盒

Free Fatty Acid Quantification Colorimetric/Fluorometric Kit


游離脂肪酸，簡稱：FFA，存在于人體內的脂質，大致可以分為膽固醇、中性脂肪（三酸甘油脂）、磷脂質、游離脂肪酸等4種。游離脂肪酸是中性脂肪分解成的物質。當肌肉活動所需能源--肝糖耗盡時，脂肪組織會分解中性脂肪成為游離脂肪酸來充當能源使用。所以，游離脂肪酸可說是進行持久活動所需的物質。


Kit Summary:
? Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
? Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids
? Species reactivity- Mammalian
? Application- The kit has a detection limit 2 μM free fatty acid in variety samples.

Features & Benefits:
? Simple procedure; takes ~40 minutes
? Fast and convenient
? Kit contains all necessary reagents for rapid, sensitive and accurate measurement of Adipolysis in cell culture samples

Kit components:
? Fatty Acid Assay Buffer
? Fatty Acid Probe in DMSO
? ACS Reagent
? Enzyme Mix
? Enhancer
? Palmitic Acid Standard (1nmol/μl)

Description:
Fatty Acids play very important roles in normal metabolism and many disease developments. They are precursors to a number of bioactive classes of compounds such as prostaglandins, leucotrienes and others, and have been implicated in diverse functions such as autism, immune system and inflammation response. BioVision’s Free Fatty Acid Quantification Kit provides a convenient, sensitive enzyme-based method for detecting the long-chain free fatty acids in various biological samples, such as serum, plasma and other body fluids, food, growth media, etc. In the assay, Fatty Acids are converted to their CoA derivatives, which are subsequently oxidized with the concomitant generation of color or fluorescence. C-8 (octanoate) and longer fatty acids can then be easily quantified by either colorimetric (spectrophotometry at λ = 570 nm) or fluorometric (at Ex/Em = 535/587 nm) methods with detection limit 2 μM free fatty acid in variety samples.

USAGE: For Research Use Only! Not For Use in Humans.

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